Can we refine the understanding of fundamental issues such as reptation and transport in crowded media, using the huge and semiflexible biomacromolecules, actin filaments?
Can we elucidate cargo transport in living cells with nm resolution?
Can we elucidate gene delivery and cellular uptake with nm resolution?
We work to answer these questions by single molecule/particle tracking experiments and statistical analysis.
Two-photon scanning microscope images of epithelial cells (Marc 145) labeled with the fluorescent dye, Laurdan. The focus is at the basal side of the cell. The cells are in interphase. The color is coded according to the phase state of membrane: liquid order (red) and liquid disorder (blue). The image area is 65µm×65µm.
Bo Wang, Stephen M. Anthony, Sung Chul Bae, and Steve Granick, "Anomalous Yet Brownian," Proc. Nat. Acad. Sci. USA. 106, 15160 (2009). [PDF; supporting information]. [In the news].
Bo Wang, Juan Guan, Stephen M. Anthony, Sung Chul Bae, Kenneth S. Schweizer, and Steve Granick, "Confining Potential When a Biopolymer Filament Reptates," Phys. Rev. Lett. 104, 118301 (2010) [PDF]. [In the news].